Brightness illusions drive a neuronal response in the primary visual cortex under top-down modulation.

Brightness illusions are a powerful tool in studying vision, yet their neural correlates are poorly understood. Based on a human paradigm, we presented illusory drifting gratings to mice. Primary visual cortex (V1) neurons responded to illusory gratings, matching their direction selectivity for real gratings, and they tracked the spatial phase offset between illusory and real gratings. Illusion responses were delayed compared to real gratings, in line with the theory that processing illusions requires feedback from higher visual areas (HVAs). We provide support for this theory by showing a reduced V1 response to illusions, but not real gratings, following HVAs optogenetic inhibition. Finally, we used the pupil response (PR) as an indirect perceptual report and showed that the mouse PR matches the human PR to perceived luminance changes. Our findings resolve debates over whether V1 neurons are involved in processing illusions and highlight the involvement of feedback from HVAs.

Overlapping yet dissociable contributions of superiority illusion features to Ponzo illusion strength and metacognitive performance.

Humans are typically inept at evaluating their abilities and predispositions. People dismiss such a lack of metacognitive insight into their capacities while even enhancing (albeit illusorily) self-evaluation such that they should have more desirable traits than an average peer. This superiority illusion helps maintain a healthy mental state. However, the scope and range of its influence on broader human behavior, especially perceptual tasks, remain elusive. As belief shapes the way people perceive and recognize, the illusory self-superiority belief potentially regulates our perceptual and metacognitive performance. In this study, we used hierarchical Bayesian estimation and machine learning of signal detection theoretic measures to understand how the superiority illusion influences visual perception and metacognition for the Ponzo illusion. Our results demonstrated that the superiority illusion correlated with the Ponzo illusion magnitude and metacognitive performance. Next, we combined principal component analysis and cross-validated regularized regression (relaxed elastic net) to identify which superiority components contributed to the correlations. We revealed that the "extraversion" superiority dimension tapped into the Ponzo illusion magnitude and metacognitive ability. In contrast, the "honesty-humility" and "neuroticism" dimensions only predicted Ponzo illusion magnitude and metacognitive ability, respectively. These results suggest common and distinct influences of superiority features on perceptual sensitivity and metacognition. Our findings contribute to the accumulating body of evidence indicating that the leverage of superiority illusion is far-reaching, even to visual perception.

The Beep-Speed Illusion Cannot Be Explained With a Simple Selection Bias.

An object appears to move at higher speed than another equally fast object when brief nonspatial tones coincide with its changes in motion direction. We refer to this phenomenon as the beep-speed illusion (Meyerhoff et al., 2022, Cognition, 219, 104978). The origin of this illusion is unclear; however, attentional explanations and potential biases in the response behavior appear to be plausible candidates. In this report, we test a simple bias explanation that emerges from the way the dependent variable is assessed. As the participants have to indicate the faster of the two objects, participants possibly always indicate the audio-visually synchronized object in situations of perceptual uncertainty. Such a response behavior potentially could explain the observed shift in perceived speed. We therefore probed the magnitude of the beep-speed illusion when the participants indicated either the object that appeared to move faster or the object that appeared to move slower. If a simple selection bias would explain the beep-speed illusion, the response pattern should be inverted with the instruction to indicate the slower object. However, contrary to this bias hypothesis, illusion emerged indistinguishably under both instructions. Therefore, simple selection biases cannot explain the beep-speed illusion.

Sanford's L dissected: A partial replication and extension of Cai et al. (2017).

Partial replications of experiments reported by Cai et al. (Attention, Perception, & Psychophysics, 79(4), 1217-1226, 2017) on the so-called Horizontal-vertical illusion confirmed that dissecting L-figures into two separate lines yields greater overestimation of (near-)verticals than do intact Ls. However, contrary to Cai et al.'s findings, which had been obtained with a staircase procedure, with the method of constant stimuli, the amount of illusion was much smaller. This divergence is explained by the self-reinforcing nature of adjustment procedures. Another finding, already reported by Cormack and Cormack (Perception & Psychophysics, 16(2), 208-212, 1974), that obtuse angles between an L's lines yield greater bias than acute angles, was also replicated in one experiment but tended to be reversed in another. Mixing dissected, upright and top-down inverted Ls and laterally oriented Ts, both with tilted lines, within one experiment confirmed that the bias for Ts is opposite to the one for Ls: For Ts, the effect of (virtual) bisection dominates, yielding an overestimation of the length of the undivided line, whereas for Ls, the horizontal-vertical anisotropy dominates, yielding an overestimation of the length of the vertical line. The differential gap effects can possibly be explained by interactions within the neural substrate between orientation-sensitive and end-inhibited neurons, and the method effects by perceptual learning.

Intrinsic excitability of human right parietal cortex shapes the experienced visual size illusions.

Converging evidence has found that the perceived visual size illusions are heritable, raising the possibility that visual size illusions might be predicted by intrinsic brain activity without external stimuli. Here we measured resting-state brain activity and 2 classic visual size illusions (i.e. the Ebbinghaus and the Ponzo illusions) in succession, and conducted spectral dynamic causal modeling analysis among relevant cortical regions. Results revealed that forward connection from right V1 to superior parietal lobule (SPL) was predictive of the Ebbinghaus illusion, and self-connection in the right SPL predicted the Ponzo illusion. Moreover, disruption of intrinsic activity in the right SPL by repetitive transcranial magnetic stimulation (TMS) temporally increased the Ebbinghaus rather than the Ponzo illusion. These findings provide a better mechanistic understanding of visual size illusions by showing the causal and distinct contributions of right parietal cortex to them, and suggest that spontaneous fluctuations in intrinsic brain activity are relevant to individual difference in behavior.

Deconfounded and mixed-symmetry versions of the Ponzo illusion figure.

One of the original Ponzo illusion figures, which consists of two converging lines between which two parallel lines of similar length have been inserted orthogonal to the figure's axis of mirror symmetry, was itself mirror-reflected so that the overall shape of the figure became "< >" or "> <", and one line at a time was inserted into each half. The usual illusion - the overestimation of the length of a line that is nearer to a vertex than a farther-away comparison line - occurred. Experiments 1 and 2 used different distances of target and comparison lines to the vertices, but identical distances of these lines from the converging lines, and so, as a tandem, deconfounded the two variables. Experiments 3 and 4 changed the symmetries of the modified Ponzo figure by reducing opposing half-angles of the converging lines or by tilting target and comparison lines concordantly or discordantly. The first measure, which created unequal distances of the endpoints of the target and comparison lines from the converging lines, hardly affected the amount of illusion. The second measure often attenuated the illusion - equally so for concordant and discordant tilts - suggesting that global and local symmetries of the stimuli, and their accordance, were less important than the vertical versus oblique orientation of target and comparison lines. Descriptively, the main cause of the Ponzo illusion seems to be the size of the gap between target and converging lines. The neural substrate of the effect may be interactions between orientation-sensitive and end-inhibited neurons.

Budgerigars (Melopsittacus undulatus) perceive the Müller-Lyer illusion.

A Müller-Lyer figure consists only of a line and arrowheads located at both ends of the line. Many comparative studies have reported that animals perceive Müller-Lyer illusion as humans, but few have used appropriate experimental designs to verify whether animal subjects actually respond to line length alone. The present study investigated whether budgerigars (Melopsittacus undulatus) can perceive the Müller-Lyer illusion by using a method that addresses this problem. Four budgerigars were trained to select a long or short line (counterbalanced across subjects) from two horizontal lines. Next, the same task was conducted using two lines, one of which was situated between arrowheads pointing either right (>>) or left (<<). In the final training phase, the arrowheads were replaced with those pointing inward (><) or outward (<>). The performance of each subject toward each stimulus set of these trainings suggested that they did not determine the length of the line by including the arrowheads. In the test phase, response tendencies to the four figures were compared. Results suggested that budgerigars perceive the Müller-Lyer illusion in the same direction as humans; however, its magnitude is larger than that of humans. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Interrelations between the Oppel-Kundt- and the T-illusion.

In order to investigate interrelations between the Oppel-Kundt- and the T-illusion, T-type figures, comprised of one dotted and one empty line (demarcated by its endpoints), separated by a gap of variable size, and rotated to oblique orientations, were judged with regard to the lengths of the two extents. The T-illusion (overestimation of the length of the undivided line) was greater for a T with a dotted undivided line and a small gap. When the divided line was dotted, the illusion vanished at a small gap and reversed at a larger one. Findings are interpreted to mirror activities of a neural T-schema as well as orientation- and density-sensitive neurons.

The color phi phenomenon: Not so special, after all?

We show how anomalous time reversal of stimuli and their associated responses can exist in very small connectionist models. These networks are built from dynamical toy model neurons which adhere to a minimal set of biologically plausible properties. The appearance of a "ghost" response, temporally and spatially located in between responses caused by actual stimuli, as in the phi phenomenon, is demonstrated in a similar small network, where it is caused by priming and long-distance feedforward paths. We then demonstrate that the color phi phenomenon can be present in an echo state network, a recurrent neural network, without explicitly training for the presence of the effect, such that it emerges as an artifact of the dynamical processing. Our results suggest that the color phi phenomenon might simply be a feature of the inherent dynamical and nonlinear sensory processing in the brain and in and of itself is not related to consciousness.

The perception of interpersonal distance is distorted by the Müller-Lyer illusion.

There is growing interest in how human observers perceive social scenes containing multiple people. Interpersonal distance is a critical feature when appraising these scenes; proxemic cues are used by observers to infer whether two people are interacting, the nature of their relationship, and the valence of their current interaction. Presently, however, remarkably little is known about how interpersonal distance is encoded within the human visual system. Here we show that the perception of interpersonal distance is distorted by the Müller-Lyer illusion. Participants perceived the distance between two target points to be compressed or expanded depending on whether face pairs were positioned inside or outside the to-be-judged interval. This illusory bias was found to be unaffected by manipulations of face direction. These findings aid our understanding of how human observers perceive interpersonal distance and may inform theoretical accounts of the Müller-Lyer illusion.

A genome-wide association study reveals a substantial genetic basis underlying the Ebbinghaus illusion.

The Ebbinghaus illusion (EI) is an optical illusion of relative size perception that reflects the contextual integration ability in the visual modality. The current study investigated the genetic basis of two subtypes of EI, EI overestimation, and EI underestimation in humans, using quantitative genomic analyses. A total of 2825 Chinese adults were tested on their magnitudes of EI overestimation and underestimation using the method of adjustment, a standard psychophysical protocol. Heritability estimation based on common single nucleotide polymorphisms (SNPs) revealed a moderate heritability (34.3%) of EI overestimation but a nonsignificant heritability of EI underestimation. A meta-analysis of two phases (phase 1: n = 1986, phase 2: n = 839) of genome-wide association study (GWAS) discovered 1969 and 58 SNPs reaching genome-wide significance for EI overestimation and EI underestimation, respectively. Among these SNPs, 55 linkage-disequilibrium-independent SNPs were associated with EI overestimation in phase 1 with genome-wide significance and their associations could be confirmed in phase 2 cohort. Gene-based analyses found seven genes to be associated with EI overestimation at the genome-wide level, two from meta-analysis, and five from classical two-stage analysis. Overall, this study provided consistent evidence for a substantial genetic basis of the Ebbinghaus illusion.

The Developing Bodily Self: How Posture Constrains Body Representation in Childhood.

Adults' body representation is constrained by multisensory information and knowledge of the body such as its possible postures. This study (N = 180) tested for similar constraints in children. Using the rubber hand illusion with adults and 6- to 7-year olds, we measured proprioceptive drift (an index of hand localization) and ratings of felt hand ownership. The fake hand was either congruent or incongruent with the participant's own. Across ages, congruency of posture and visual-tactile congruency yielded greater drift toward the fake hand. Ownership ratings were higher with congruent visual-tactile information, but unaffected by posture. Posture constrains body representation similarly in children and adults, suggesting that children have sensitive, robust mechanisms for maintaining a sense of bodily self.

An Optical Illusion Pinpoints an Essential Circuit Node for Global Motion Processing.

Direction-selective (DS) neurons compute the direction of motion in a visual scene. Brain-wide imaging in larval zebrafish has revealed hundreds of DS neurons scattered throughout the brain. However, the exact population that causally drives motion-dependent behaviors-e.g., compensatory eye and body movements-remains largely unknown. To identify the behaviorally relevant population of DS neurons, here we employ the motion aftereffect (MAE), which causes the well-known "waterfall illusion." Together with region-specific optogenetic manipulations and cellular-resolution functional imaging, we found that MAE-responsive neurons represent merely a fraction of the entire population of DS cells in larval zebrafish. They are spatially clustered in a nucleus in the ventral lateral pretectal area and are necessary and sufficient to steer the entire cycle of optokinetic eye movements. Thus, our illusion-based behavioral paradigm, combined with optical imaging and optogenetics, identified key circuit elements of global motion processing in the vertebrate brain.

The effect of the Müller-Lyer configuration on saccadic eye movements is not fully due to illusory perception.

Previous research has shown that both perception and oculomotor control are affected by visual illusions. While these findings appear to suggest a common code of visual processing for perception and oculomotor control, there remains the possibility that the perceptual and the oculomotor effects emerge through partially different processes. In three experiments, we replicated the previous finding that perception and saccades were both biased by the typical Müller-Lyer configurations. However, using a non-Müller-Lyer setup in which the perceptual illusion effect was much restrained, we did not observe a comparable reduction in the saccadic effect. Instead, the saccadic effect by Müller-Lyer configuration could be partially due to the center-of-gravity (CoG) effect (i.e., the tendency for saccades to land at the center of gravity of the stimuli). These results indicate that the influence of the Müller-Lyer configuration on saccadic eye movements is a mixed effect of perceptual representation and CoG, rather than exclusively due to the illusory perception. We further found that the saccadic and perceptual effects were not correlated at the trial-by-trial level, which suggest that there could be largely independent sources of noise for perception and saccadic control.NEW & NOTEWORTHY The Müller-Lyer illusion affects both perception and oculomotor control, but it is unknown whether these effects arise from the same or different underlying mechanisms. We developed a modified version of the Müller-Lyer configuration, which largely reduced the perceptual illusion effect compared with the typical configuration but reduced the saccadic effect to a much less extent. Such difference indicates that influence of the Müller-Lyer configuration on saccadic eye movements is not fully mediated by illusory perception.

Adaptation to feedback representation of illusory orientation produced from flash grab effect.

Adaptation is a ubiquitous property of sensory systems. It is typically considered that neurons adapt to dominant energy in the ambient environment to function optimally. However, perceptual representation of the stimulus, often modulated by feedback signals, sometimes do not correspond to the input state of the stimulus, which tends to be more linked with feedforward signals. Here we investigated the relative contributions to cortical adaptation from feedforward and feedback signals, taking advantage of a visual illusion, the Flash-Grab Effect, to disassociate the feedforward and feedback representation of an adaptor. Results reveal that orientation adaptation is exclusively dependent on the perceived rather than the retinal orientation of the adaptor. Combined fMRI and EEG measurements demonstrate that the perceived orientation of the Flash-Grab Effect is indeed supported by feedback signals in the cortex. These findings highlight the important contribution of feedback signals for cortical neurons to recalibrate their sensitivity.

Dependence of the filled-space illusion on the size and location of contextual distractors.

For most observers, the part of the stimulus that is filled with some visual elements (e.g., distractors) appears larger than the unfilled part of the same size. This illusion of interrupted spatial extent is also known as the \'filled­space' or 'Oppel­Kundt' illusion. Although the continuously filled­space illusion has been systematically studied for over a century, there is still no generally accepted explanation of its origin. The present study aimed to further develop our computational model of the continuously filled­space illusion and to examine whether the model predictions successfully account for illusory effects caused by distracting line­segments of various lengths that are\r\nattached to different endpoints (i.e., terminators) of the reference spatial interval of the three­dot stimulus. Our experiments confirm that the illusion manifests itself along a distracting segment located both inside and outside of the reference interval. In the case of two distractors arranged symmetrically with respect to the lateral terminator, we found that the magnitude of the illusion is approximately equal to the sum of the relevant values obtained with separate distractors. The results of experiments using vertical shifts of distractors supported the model's assumption regarding the two­dimensional Gaussian profile of hypothetical areas of weighted spatial summation\r\nof neural activity. A good correspondence between the experimental and theoretical results supports the suggestion that perceptual positional biases associated with the context­evoked increase in neural excitation may be one of the main causes of the continuously filled­space illusion.

Perceptual rivalry across animal species.

This review in memoriam of Jack Pettigrew provides an overview of past and current research into the phenomenon of multistable perception across multiple animal species. Multistable perception is characterized by two or more perceptual interpretations spontaneously alternating, or rivaling, when animals are exposed to stimuli with inherent sensory ambiguity. There is a wide array of ambiguous stimuli across sensory modalities, ranging from the configural changes observed in simple line drawings, such as the famous Necker cube, to the alternating perception of entire visual scenes that can be instigated by interocular conflict. The latter phenomenon, called binocular rivalry, in particular caught the attention of the late Jack Pettigrew, who combined his interest in the neuronal basis of perception with a unique comparative biological approach that considered ambiguous sensation as a fundamental problem of sensory systems that has shaped the brain throughout evolution. Here, we examine the research findings on visual perceptual alternation and suppression in a wide variety of species including insects, fish, reptiles, and primates. We highlight several interesting commonalities across species and behavioral indicators of perceptual alternation. In addition, we show how the comparative approach provides new avenues for understanding how the brain suppresses opposing sensory signals and generates alternations in perceptual dominance.

Local biases drive, but do not determine, the perception of illusory trajectories.

When a dot moves horizontally across a set of tilted lines of alternating orientations, the dot appears to be moving up and down along its trajectory. This perceptual phenomenon, known as the slalom illusion, reveals a mismatch between the veridical motion signals and the subjective percept of the motion trajectory, which has not been comprehensively explained. In the present study, we investigated the empirical boundaries of the slalom illusion using psychophysical methods. The phenomenon was found to occur both under conditions of smooth pursuit eye movements and constant fixation, and to be consistently amplified by intermittently occluding the dot trajectory. When the motion direction of the dot was not constant, however, the stimulus display did not elicit the expected illusory percept. These findings confirm that a local bias towards perpendicularity at the intersection points between the dot trajectory and the tilted lines cause the illusion, but also highlight that higher-level cortical processes are involved in interpreting and amplifying the biased local motion signals into a global illusion of trajectory perception.

Opposite effects of motion dynamics on the Ebbinghaus and corridor illusions.

We recently showed that motion dynamics greatly enhance the magnitude of certain size contrast illusions, such as the Ebbinghaus and Delboeuf illusions. Here, we extend our study of the effect of motion dynamics on size illusions through a novel dynamic corridor illusion, in which a single target translates along a corridor background. Across three psychophysical experiments, we quantify the effects of stimulus dynamics on the Ebbinghaus and corridor illusions across different viewing conditions. The results revealed that stimulus dynamics had opposite effects on these different classes of size illusions. Whereas dynamic motion enhanced the magnitude of the Ebbinghaus illusion, it attenuated the magnitude the corridor illusion. Our results highlight precision-driven weighting of visual cues by neural circuits computing perceived object size. This hypothesis is consistent with observations beyond size perception and may represent a more general principle of cue integration in the visual system.